Sinomenine inhibits brain nuclear factor-κB activity in rat experimental autoimmune encephalomyelitis
Abstract：Objective To investigate the therapeutic effects of sinomenine on the activity of brain nuclear factor-κB (NF-κB) rat in experimental autoimmune encephalomyelitis (EAE). Methods EAE rats were induced by immunization of female Wistar rats with brain and spinal cord homogenate followed by treatment with or without sinomine. The severity of EAE rats was evaluated by clinical scores and histological examination. The activity of brain NF-κB and the plasma cortisol were determined by ELISA kits. Plasma prolactin was assessed by the RIA kit. Results The brain NF-κB binding activity was increased significantly in EAE rats. The treatment of sinomenine reduced the clinical scores of EAE significantly, decreased the number of inflammatory foci in the central nervous system, and inhibited the activation of brain NF-κB in EAE rats. Besides, the level of plasma cortisol was increased and the level of plasma prolactin was decreased after the treatment of sinomenine.Conclusion Inhibiting brain NF-κB binding activity could be a mechanism of sinomenine protecting of rats from EAE, and the inhibition of NF-κB is related to the alteration of cortisol and prolactin in vivo.
Keywords：SinomenineExperimental autoimmune encephalomyelitisNuclear Factor-κBCortisolProlactin
Publication Date：2005-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )