Isolation and purification of soluble typeⅡcollagen with FPLC system
Abstract：Objective To isolate and purify soluble type Ⅱcollagen by FPLCsystem． Methods Xiphoid cartilage of chickens was digested with pepsin and was centrifuged to obtain the rough protein solution． Then， FPLC system was adopted to carry on DEAE ion exchange chromatography and desalting chromatography in order to purify the protein． Results It was found that the bands of CⅡsamples and standard CⅡwere at the same location by SDS-PAGE． Conclusions The improved method we adopted had significant advantages such as the high purification efficiency，excellent stability and the fast and simple working process compared with traditional method．
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 228-230 )