Screening for washing condition in solid-phase R-phycoerythrin fluorescence immunoassay
Abstract：Objective To screen for the optimal washing buffer inR-phycoerythrin fluorescence immunoassay.Methods Change the ionic intensity and pH value of the washing buffer． Screen the washing buffer by comparing the checkout ratio of positive samples and the pseudo-positive ratio of negative samples．Results Increasing ionic intensity can be helpful to decrease pseudopositive phenomenon in negative samples and to increase sensitivity when concentration is below 120 mmol／L． Pseudopositive ratio can be decreased to zero． when concentration is near to 120 mmol／L and the positive checkout ratio can be up to 93．3％． But much higher concentrations may restrict the sensitivity to be increased． A weak basic washing condition is appropriate．Conclusion 120 mmol／L PBS （pH7．5） is suitable for R-phycoerythrin fluorescence immunoassay both in the diagnosis specificity and in the sensitivity．
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 219-221 )