Cloning and sequence analysis of cDNA coding for group Ⅰ allergen of dermatophagoides farinae(Der f Ⅰ)
Abstract：Objective To acquire the cDNA of group I allergen of (Der f Ⅰ) ofGuangzhou, China for further usage in construction of DNA vaccine and expression of the recombinant protein. Methods The live mites of local area which had been identified and cultured were picked. The total RNA was extracted. The Der f Ⅰ cDNA was amplified with RT-PCR. Then it was subcloned into a vector and sequenced. Results The segment with 632 bases was determined. The sequence homology with the published one （emb｜X65196.1｜）on gene bank was 99% at the nucleotide level.The deduced amino acid sequence homology was 100%. Conclusion It is the first time that we achieve the cDNA of Der f Ⅰ of Guangzhou. Its sequence homology is high as compared with the published one.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 213-215 )