Effects of protein-overload on the expression of CD54 and vimentin in murine renal tubular epithelial cell line
Abstract：Objective To explore the expressions of CD54 (IntercellularAdhension Molecule-1) and cytoskeleton intermediate filament protein vimentin in murine renal tubular epithelial cell line with the effect of protein-overload. Methods One group of renal proximal tubular epithelial cell line NRK52E was treated with IgG(10 mg/mL) or different concentrations of BSA(1,15,30 mg/mL) ,another group served as control. The expression of CD54 was identified respectively by flow cytometry (FACS). Vimentin protein expression on the slides of cells treated with or without IgG or BSA was measured by immunohistological staining. Results The expression of CD54 increased with the effect of IgG by 40.1% (P<0.05),and increased with BSA(15 or 30 mg/mL) by 24.8% or 25.0% without statistical significant difference. IgG and BSA(15,30mg/mL) could stimulate the expression of vimentin compared with control group (P<0.05). Conclusion Protein-overload could cause the increase of adhension molecule's expression and the change of cytoskeleton in murine renal epithelial cell line, so it may be an important factor in the process of tubular interstitial infiltration and scarring.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 204-206 )