Generation of dendritic cells from human bone marrow and efficient presentation of tumor antigen associated with Epstein-barr virus
Abstract：Objective To expand dendritic cells (DCs)from human bone marrowand design vaccine against cancer concerned with the infection of EBV，for instance，Burkitt's lymphoma、nasopharyngeal cancer、Hodgkin's disease and so on in vitro. Methods CD34+ hematopoietic stem cells were isolated from normal bone marrow by mini-MACS.Then CD34+ hematopoietic stem cells were cultured in hGM-CSF、hTNF-α、hIL-3、hIL-4 for two weeks. T cells stimulating activity was determined by allo-MLR. Results These cells expressing high levels of CD1αhave typical dendritic morphology. They could acquire Epstein-Barr virus latent membrane glycoprotein gp340 efficiently and induce T cells increasing stimulatory capacity in MLR. Conclusion We could obtain large amount of mature DCs by in vitro culture of human CD34+ hematopoietic stem cells. It will probably become a potent approach of gp340-loaded DC against tumor in relation to EBV.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 192-195 )