Clone and sequence analysis of human Helicobacter pylori heat shock protein A gene
Abstract：Objective To obtain DNA of human Helicobacter pylori (Hp) heatshock protein A(hspA), and investigate the nucleotide sequence. Methods The HspA DNA was amplified by PCR from the chromosomal DNA of clinical isolated Helicobacter pylori and inserted into PinPointTMXa-3.Then the sequence was analysed. Results The HspA gene's nucleotide homology was 95.8% as compared to that in Genebank. Conclusion A confirmed HspA gene has been obtained, thus a good foundation for recombination, expression and associated research laid.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 185-188 )