Immunogenicity of two bivalent Shigella v accines after intranasal or intragastric administration in mice
Abstract：Objective To observe the effect of two different mucosaladministration routes to the immunogenicity of two bivalent Shigella vaccines. Methods BALB/c mice were divided into 3 groups, 20 mice per group. Mice were immunized respectively with Ipa+ or Ipa－ vaccines 3 times with an interval of 2 weeks by intranasal or intragastric route.The serum, nasopharynx wash, lung wash, small intestinal wash and vaginal wash were collected. The amounts of IgA and IgG against flexineri and sonnei LPS were measured by ELISA. Results The levels of specific antibody in nasopharynx wash, lung wash, small intestinal wash，vaginal wash and serum were significantly increased after intranasal immunization with 4×107 CFU of two bivalent Shigella vaccines.When the dosage increased 20 or 200 times,the levels of specific antibody in nasopharynx wash,lung wash,small intestinal wash,vaginl wash and serum were significantly increased after intragastric immunization．Conclusions Two bivalent Shigella vaccines can induce system and several local mucosal immunity reaction by nasal mucosa administration routes in low dosage compared with intragastric route(about 1/20).
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 181-184 )