Relationship between cytoplasmic domain of leukemia inhibitory factor receptor and STAT3 activation in HL-60 cell
Abstract：Objective To investigate the relation of each subunit of leukemiainhibitory factor(LIFR)and STAT3 in HL-60 for studying the mechanism of leukemia cell proliferateation disorder. Methods We constructed the chimerical receptor by exchanging the cytoplasmic domain of gp130 and gp190 (130/190,190/130)and expressed it on the membrane of HL-60. Rapid tyrosine phosphorylation of STAT3 and STAT3 level in HL-60 was detected by means of immunoblotting and immunobiochemistry. Results As compared with the group of wild type receptor,higher level of STAT3 expression and rapid phosphorylated STAT3 was found in group of 130/190(P＜0.01). We also found that expression of rapid phosphorylated STAT3 in group of 130/190 had a time-dependent manner. Conclusion The result of this study suggest that the cytoplasmic domain of gp190, not gp130, participats in the induction of STAT3 activation in HL-60.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 177-180 )