Effects of hp450RAI plasmid transfection on cellular function and IFN-α expression of HL-60 cells
Abstract：Objective To investigate the effects of human cytochrome P450enzyme gene(hp450RAI)transfer on retinoic acid metabolism and cellular proliferation in HL-60 cells.Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study. Results It was shown that the hp450RAI mRNA was not induced by the treatment of 2.5×10－7 mol/L all-trans retinoic acid(ATRA) in HL-60 cells; The hp450RAI gene transfer made the HL-60 cells express hp450RAI greatly, which did not affect significantly the cell growth and metabolism of ATRA in HL-60 cells, but the use of Ketoconazole or IFN-α could cut down the metabolic rate of ATRA in HL-60 cells,and enhance the growth inhibitory induced by ATRA. Conclusion The hp450RAI may not be the key cytochrome P450 enzyme to modulate the metabolism of retinoic acid in wild HL-60 cells. The hp450RAI inhibitor ketoconazole or IFN-α can cooperate with ATRA to inhibit the growth of HL-60 cells, which may partly owe to the low metabolism rate of ATRA and over-expression of IFN-α in HL-60 cells.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 173-176 )