Study on ConA promoting phagocytosis, cytotoxicity and producing effectors of macrophages
GAO Li-fen
Liu Jun-yan
TANG Hong-ming
QU Xue-ju
JIANG Zheng-ming
Abstract：Objective To study how ConA actives macrophages in vivo to produce cytotoxic effectors and its phagocytic functions,and cytotoxicity. Methods ConA was intraperitoneally injected(ip). Cock red blood cells(cRBC) were used to evaluate MΦ phagocytic activity,and S180 cells as target cells to analyze MΦ dependent cytotoxicity(MTC).Nitric oxide(NO),TNF-α and IL-1 levels of MΦ cultural supernatant were measured using griess reagent,L929 cells MTT method and thymocytes proliferation test respectively. Results ConA could promote MΦ to phagocytize cRBC and kill S180 cells,enhance the production of such factors as NO,TNF-α and IL-1 by MΦ. There was significant difference compared with PBS control group(P＜0.01). Conclusions ConA could stimulate MΦ to produce effectors, which mediate immune regulation of ConA to MΦ.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 110-112 )