Recombination and expression of a component of urease B subunit transmembrane protein of helicobacter pylori
MAO Xu-hu
LU Dong-shui
GUO Hong
WU Chao
WANG Fu-kun
ZOU Quan-ming
Abstract：Objective To recombine and express a component of urease B subunit transmembrane protein of helicobacter pylori. Methods A 732 bp gene fragment of urease B subunit of helicobacter pylori was cloned into pET11C and transformed into BL21（DE3）E.coli. The positive clone was induced with IPTG. The expression of target protein was analysed by SDS-PAGE and Western blot. Results It is successful to construct the recombinant plasmid pET-UreB0.7 containing urease B subunit 0.7 kb gene fragment. A protein （MW≈28 000 u） with immunoreactivity， was expressed by 19.8％ in BL21（DE3）E.coli induced with IPTG. Conclusions The recombinant component of urease B subunit transmembrane protein may play a role in the research of its biological function and might be used as the vaccine against helicobacter pylori.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 94-96 )