Dot immunogold filtration assay for detection of anti-sperm antibody in serum of infertile couples
Abstract：Objective Using dot immunogold filtration assay（DIGFA） toestablish a new method for detecting anti-sperm antibody in serum of infertile couples.Methods A dot immunogold filtration assay was developed by coating purified sperm membrane antigen on nitrocellulose membrane， labeling SPA with colloidal gold， the whole procedure could be finished in 2-4 min.Results The coincidence rate of the results of simultaneous determination of serum samples with ELISA and DIGFA was 97.7％.DIGFA had an overall specificity and sensitivity of 96.2％（TN／TN＋FP×100％） and 88.9％（TP／TP＋FN×100％），positive predictive value and negative predictive value of 84.2％ and 97.2％ respectively.Conclusions Using DIGFA to detect anti-sperm antibody in human serum is more simple and rapid， and is of value for clinical application.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 64-65 )