Effect of recombinant interferon-γ（rIFN-γ）on IL-8 and IL-10 released by peripheral blood mononuclear cells（PBMC）in asthmatic patients
Abstract：Objective To study the effect of （rIFN-γ）on IL-8 and IL-10release in asthmatic patients PBMC and its relationship with serum level of ECP．Methods Samples of PBMC obtained from 8 cases of asthmatic patients and five cases of normal persons were cultured in RPMI 1640 containing 10％ bovine serum．After 66 hours，the supernatants of cultured PBMC of asthmatic patients were divided into three groups：group A （rIFN-γ），group B （Dexamethasone）and group C （control group）．Concentration of IL-8 and IL-10 in the supernatant in the three groups was determined after 72 hours．Serum ECP levels were also determined．Results The serum ECP level was positively and signifocantly correlated with content of IL-8 in the supernatant （r＝0.701）and negatively correlated With that of IL-10（r＝0.638）．There was significant difference between the serum ECP level and the IL-8 and IL-10 concentration in supernatants of cultured PBMC （P＜0.05）．After rIFN-γ intervention ，the IL-8 level decreased significantly （P＜0.05）and the IL-10 increased singificantly（P＜0.05）than that of control group．There was no significant difference after dexamethasone intervention．Conclusion rIFN-γ may decrease the level of IL-8 which had anti-inflammation effects．IL-10 may inhibit the proliferation and differentiation of inflammatory cells caused by antigen stimulation and may inhibit the synthesis of cytokines and inflammatory mediators and also inhibit B cells to secret IgE．rIFN-γ can promote the synthesis and secretion of IL-10 which can increase its anti-inflammatory function．
Machine-generated Keywords：
Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 50-52 )