Effect of immunogenicity of liver cancer cell lines by transduction of adenovirus-mediated human B7-1 gene
Abstract：Objective To investigate the change of immunogenicity of livercancer cells transduced with adenovirus-mediated human 4 kind of genes (p53，GM-CSF，B7-1，IL-2)．Methods Using human mixed lymphocyte of peripheral blood and tumor cell coculture，cytotoxicity assay of peripheral blood T lymphocyte，we studied the change of immunogenicity of liver cancer cells transduced with Ad-multigenes．Results The proliferation of human peripheral blood T lymphocytes and T lymphocyte cytotoxicity activity against parent tumor cells were increased by transduction of Ad-multigenes in liver cancer cells HepG2 and BEL-7402．IL-12 has a strong synergetic effect with Ad-multigenes in inducing proliferation and cytotoxicity of T cells．Conclusion The immunogenicity of liver cancer cells was enhanced after transduction of adenovirus-mediated multigenes which containing B7-1 molecule．
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 34-36 )