Endothelial cell-targeted expression of human tumor necrosis factor α mutant with higher activity from retroviral vectors
Abstract：Objective To develop a new approach in specific gene therapy oftumor.Methods TNF-α D11a was inser-ted downstream of KDR promoter in retroviral vector pLXSN in which 299 bp of 3’ LTR had been deleted. The constructed retroviral vectors pLXSN-D299-KDRp-TNF-α D11a were transfected into PA317 packaging cells by lipofectamine reagent. Then the recombinant retroviruses were used to infect endothelial cells and NIH3T3 cells. Expression and bioactivity of TNF-α D11a in culture medium of endothelial cells were identified by ELISA and MTT， respectively. Results Retroviral vectors， carrying TNF-α D11a and KDR promoter， were constructed successfully. Expression level of TNF-α D11a in endothelial cells was higher than that in NIH 3T3， and inhibitory effects of TNF-α D11a released from endothelial cells on tumor cells prolife-ration were also stronger than those from NIH3T3 cells.Conclusion Endothelial cell-targeted expression of TNF-α D11a was regulated by KDR promoter.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 30-33 )