Establishment of an apoptotic model on activation-induced CD4＋T lymphocytes apoptosis mediated by superantigen SEB
Abstract：Objective It is essential to establish the apoptotic model forexploration of the molecular mechanism and signaling pathways in activation-induced CD4＋T lymphocytes apoptosis mediated by superantigen SEB．Methods The model of activation-induced CD4＋T lymphocytes apoptosis by SEB was established according to electron micrograph of the apoptotic characteristics，DNA fluorescence flow cytometric profiles of PI-stained apoptotic CD4＋T cells，the appearance of the nucleosomal DNA ladder in agarose gels，and the quantitative analysis of DNA fragmentation percent by improved DPA methods．Results There were typical morphological changes of apoptosis after SEB-responsive CD4＋T lymphocytes stimulated with 4 μg／mL SEB for 12 hours．In addition，there was an apperance of apoptotic subdiploid peak，a higher side scatter and a lower forward scatter of typical apoptotic characteristics for SEB-induced apoptotic CD4＋T lymphocytes than normal control．Moreover，analysis of the agarose gel eletrophoresis revealed that there was an apperance of typically apoptotic DNA ladder for SEB-induced apoptotic CD4＋T lymphocytes．Finally，the kinetic increment of apoptosis was related to the time of stimulatation by SEB，and the increment of DNA fragmentation percent could be completely prevented by Fas-Ig fusion protein．Conclusion Our results strongly suggest that the apoptotic model might be a useful tool to explore the molecular mechansim and signaling transduction pathways in activation-induced CD4＋T lymphocytes apoptosis mediated by superantigen SEB．
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 17-21 )