Changes of sIL-2R and IFN-γ in mice induced by recombinant BCG-Sj26GST vaccine

doi:10.3969/j.issn.1000-8861.2001.01.003

Abstract：Objective To investigate the effects of recombinant BCG-Sj26GSTvaccine of schistosoma japonicum on sIL-2R and IFN-γ produced by spleen cells of mice. Methods In the first experiment， BALB／C mice were immunized subcutaneously by 1×106 and 1×108 CFU vaccine respectively， challenged with Sj cercariae 8 weeks after immunization and killed 6 weeks after infection to separate spleens， PBS served as control； in the second experiment， after immunized subcutaneously and intravenously by 1×106 CFU vaccine respectively，4 mice were killed to separate spleens on 0、4、8、10、14 and 16 weeks after immunization， spleen cells were stimulated by Sj26 or ConA. Levels of sIL-2R and IFN-γ in supernatant of spleen cells were detected by ELISA.Results Levels of sIL-2R and IFN-γ increased obviously after immunization with the vaccine against challenge with Sj cercariae； dynamic observation showed sIL-2R and IFN-γ reached the highest level on 8－10 weeks and 4－8 weeks after immunization respectively. Conclusion Recombinant BCG-Sj26GST vaccine of schistosoma japonicum may strengthen host’s Th1 response， promote Th1 cells to secrete IL-2 and IFN-γ. These cytokines and immune cells may influence each other to increase host’s protection against schistosomiasis.