Preparation of monoclonal antibodies to nuclear matrix proteins of tissues surrounding esophagus cancer and its preliminary identification
ZHANG Xin-mei
LIN Han-liang
Liu Le-he
ZHANG Chang-Qing
ZHONG Shu-ping
ZENG Jinyun
ZHENG Ke-Li
Abstract：Objective To prepare anti-nuclear matrix proteins of tissues surrounding esophagus cancer monoclonal anti-
bodies. Methods Differences among nuclear matrix proteins (NMPS) of normal esophagus tissues, esophagus cancer tissues
and tissues adjacent to cancer were identified by SDS-PAGE. Balb/c mouse were hyperimmunized with NMPS of tissues adja-
cent to esophagus cancer. Five mouse lymphocyte hybridoma cell lines were established and preliminary identification of cell
lines 9-1-2/D, 9-1-7/D were made. Results Immunohistochemical analysis showed monoclonal antibody 9-1-2/D reacted both
with esophagus cancer and normal esophageal tissues; monoclonal antibody 9-1-7/D reacted only with nucleoli of esophagus
cancer cells. Western Blotting analysis showed monoclonal antibody 9-1-2/D reacted with 52 kD NMPS of normal esophagus
tissues and tissues adjacent to esophagus cancer and reacted with 55 kD NMP of esophagus cancer tissue. Monoclonal anti-
body 9-1-7/D reacted only with 46 kD protein of esophagus cancer tissues and tissues adjacent to cancer. Conclusion Mono-
clonal antibodies prepared by us might be a new method for early diagnosis of esophagus cancer.
Keywords：tissues adjacent to esophagus cancernuclear matrixmonoclonal antibody
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 457-460 )