Detection of human cytomegalovirus antigen after renal transplantation and its clinical significance
XU Hong-xu
ZENG Wen-tao
ZHENG Ke-Li
CHEN Lian-zhou
Fu Xi
HONG Liang-qing
WANG Xiao-bo
Abstract：Objective To provide a rapid and early antigen diagnostic technique for the patients with human cy
tomegalovirus(HCMV) infection after renal transplantation and discuss its clinical significance. Methods A two-step tech-
nique of immunohistochemical EnvisionTM was developed to detect antigen in peripheral blood polymorphonuclear leucocytes
(PMNL) using HCMV monoclonal antibody of immediate early antigen(IEA) and early antigen (EA) to diagnose active infec
tion of HCMV. Results This assay was not disturbed by background and nonspecificity of stain. It is easy to distinguish posi
tive cells from negative cells. HCMV antigen positive cell were counted in every 5. 0× 104 PMNL. Among 86 renal transplan-
tation recipients, 39 were positive for HCMV antigen, the positive rate was 45.3%, the average count of positive cells were
16. 5/5. 0×104 PMNL. In patients suffering form the HCMV disease and the subclinical infection the average counts of posi-
tive cells were 24.5±18.2/5. 0×104 PMNL and 12.0±10. 6/5. 0× 104 PMNL respectively. 15 healthy people (anti-HCMV
antibody negative) were also tested with the antigen as control. The results of HCMV antigen were negative. Conclusion
This method is rapid, specific, accurate and stable for detecting HCMV antigen. The latent infection and active infection of
HCMV can be distinguished by this method. It is useful in the early diagnosis of HCMV infection and may be sued as the prin-
cipal method for guiding the immunological rejection of therapy and judging prognosis.
Keywords：renal transplantationhuman cytomegalovirusinfectionantigenimmunohistochemistry
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 454-456 )