The gene cloning and sequence of THANK
WU Dong
SHEN Feng
LOU Yong-hua
JIAO Bing-hua
WU Meng-chao
Abstract：Objective To clone and sequence the intact THANK gene and its extracelluar fragment. Methods THANK
cDNA was amplified by RT-PCR using RNA isolated from HL-60 cell lines. The fragment was linked to pMD18-T vector and
was introduced into E. coli K802. After extracting the plasmid,further sequence was performed by ABIPRISMrM377XL DNA
sequencer. Result A 858 bp DNA fragment was amplified and the cDNA sequence is identical to the published sequence en-
coding THANK gene. Conclusion THANK cDAN has been cloned successfully,thus providing the possibility of further re-
search of THANK gene.
Keywords：human THANK geneRT-PCRcDNA cloning
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 432-435 )