Upregulation of ICAM-1 expression enhances cytotoxic sensitivity of tumor cells to LAK by LTA from bifidobacterium
JIANG Hong
HU Hong
WEI Qi-ou
Abstract：Objective To investigate whether the recognizing and cytotoxic abilities of LAK can be intensified by bifi-
dobacterial lipoteichoic acid(LTA) and the possible role of ICAM-1 in this process. Methods Standard MTT assay was used to
evaluate the binding rate and cytotoxic capability of LAK to LoVo cells. Flow cytometric assay and ELISA were used to deter-
mine the expression of ICAM-1 on these cells. Results LAK cells bound much easier to LoVo cells with an increase from 9.
62% to 24.42% as well as a double increase of the anti-tumor sensitivity of LoVo cells to LAK after challenge with 50 μg/ml
LTA of Bifidobacterium bffidum 1101. Compared with the control group,both the percentage of ICAM-1 positive cells and the
amount of ICAM-1 expression on LoVo cells were greatly increased directly after the challenge of LTA. Conclusion The pos-
sible mechanism of the increase of antitumor activity lies in that Bifidobacterial LTA can intensify the binding and recognizing
capability of LAK to tumor cells by promoting the expression of ICAM-1 on the surface of LoVo cells. The therapeutic effect
on intestinal cancer may be enhanced by the combined treatment of bifidobacterial LTA and LAK.
Keywords：bifidobacteriumlipoteichoic acidICAM-1LAK
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 429-431,441 )