Inhibition of the DNA-binding activity of GATA3 and NFAT in CD4+ T lymphocytes in sensitized mice by triptolide
CHENG Xiao-rning
WANG Chang-zheng
QIAN Gui-sheng
Abstract：Objective To explore the changes of nuclear factors GATA3,NFAT activities in CD4+T lymphocytes in
sensitized mice,and the effects of triptolide (TP). Methods The peritoneal injection of ovalbumin(OVA)was used to produce
the sensitized BALB/c mouse model. Panning method was used to isolate CD4+ T lymphocytes. The DNA-binding activity of
GATA3 and NFAT in CD4+T lymphocytes by treatment with TP and dexamethasone (DM)were assayed by using elec-
trophoretic mobility shift assay(EMSA). Results There were faint activities of GATA3 and NFAT activities in CD4+T lym-
phocytes in normal controls. After stimulation with ConA,DNA-binding activities of GATA3 and NFAT in CD4+ T lympho-
cytes of sensitized mice were higher than that in the normal controls. Changes of DNA-binding activities were in a time-depen-
dent manner. TP and DM could inhibit the DNA-binding activities of GATA3 and NFAT. Conclusions GATA3 and NFAT are
important nuclear transcription factors that regulate transcription of Th2 type cytokines. Molecular mechanisms for the sup-
pressed expression of Th2 type cytokines by TP or DM may be involved in the inhibition of GATA3 and NFAT.
Keywords：triptolideCD4+ T lymphocytetranscription factor
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 417-421 )