Effect of transfection with M-CSF gene on oxidative injury-resistance of RAW264.7 cells
PANG Zhan-jun
ZHOU Mei
CHEN Yuan
XING Fu-qi
Abstract：Objective To investigate the effect of macrophage colony-stimulating factor(M-CSF)on the oxidative injury
to monocytes/macrophages. Methods Two M-CSF-overexpressed RAW264.7 cell lines were established by applying transfec-
tion method. The oxidative injurious effect of tertbutyl hydroperoxide (tbOOH) on the established cell lines was also investi-
gated. Results The two M-CSF-transfected RAW264.7 cell lines showed high capacity of secreting M-CSF. Comparing to the
non-transfected RAW264.7 ceils,the M-CSF-overexpressed RAW264.7 cells were more vulnerable to oxidative injury. Con-
clusion In some occasions,M-CSF could aggravate the oxidative injury to macrophages.
Keywords：macrophage colony-stimulating factoroxidative stressRAW264.7 cell linegene transfection
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 414-416 )