High-level expression of anti-CEA chimeric antibody in CHO-dhfrcells
RAN Yu-Liang
KONG Jian
ZHAO Ze-guo
SUN Li-xin
YU Long
LIU Jun
YANG Zhi-hua
Abstract：Objective To express chimeric anti-CEA antibody in dihydrofolate reductase-deficient Chinese hamster
ovary(CHO-dhfr) cells at high-level. Methods The eukaryotic expression vectors of chimeric anti-CEA antibody were con-
structed and inducted into CHO-dhfr- cells. High-level expression was achieved after cells adapted to methotrexate. Chimeric
antibody was characterized by ELISA,Western blot ,and radioimmunoassays in vivo. Results The cell strain that secrets anti-
CEA chimeric antibody at high-level of 60 μg/ml was established. It was found that the chimeric antibody contained constant
regions of human Ig and had the specificity against CEA by ELISA,Western blot,and radioimmunoassays in vivo. Conclusion
The anti-CEA mouse-human chimeric antibody was successfully expressed at high-level in CHO cells.
Keywords：carcinoembryonic antigenchimeric antibodyeukaryotehigh-level expressionMTX
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 407-410 )