In vitro studies on the expression of FLT3 Ligand regulated by Egr-1 regulated sequence
Abstract：Objective To explore the regulating effects of radiation inducible gene on the expression of hematopoiedtic growth factor genes. Methods The human FLT3 Ligand (FL) cDNA and EGFP cDNA were linked together with IRES and then inserted into the eukaryotic expression vector pCI-Egr, which was constructed by substituting CMV promoter in pCIneo with the Egr-1 promoter(Egr-EF). The expression of FL in HFCL/EF cells were confirmed with RT-PCR、ELISA、FACS and cell culture. Results The activity of EGFP in transfected cells increased after exposure to 2. 5Gy. The amounts of secreted FL in serum-free supernatants of HFCL/EF were significantly higher than the control group. FL cDNA was successfully expressed in the cells by ELISA and RT-PCR analysis. At day 10 of culture the number of CD34+ cells in HFCL/EF culture supernatants was significantly higher than that of non-radiation group. Conclusion These results showed that radiation can enhance the ability of the supernatants containing FL of HFCL/EF to expand early hematopoietic progenitor cells and protect hematopoietic cells from radiation-injury effects.
Keywords：hematopoietic growth factorradiation inducible genegene therapyradiationbone marrow stromal cell
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 166-171 )