Influence of retinoic acid on CDK2-overexpressing HL—60 cells
Abstract：Objective To determine the function and expression of CDK2 in the course of proliferation and differentia-tion of HL—60 cells induced by retinoic acid(ATRA)．Methods We introduced CDK2cDNA into HL—60 cells and treated them with ATRA．Results ATRA could inhibit the growth and induce differentiation of HL-60 cells overexpressing CDK2，about 80 percent cells were arrested at G0/G1 phase after 3d exposure to ATRA．Comparing with the cells without trapsfection，the effect of ATRA on the transfected HL—60 cells was not so muchas that on the normal HL-60 cells．Moreover，the protein ex-pression of CDK2 did not change dramatically after exposure to ATRA． Conclusion The results above suggest CDK2 is very important in G1→Sphase transition，it's overexpression could promote the proliferation．ATRA could inhibit the prolifera-tion and promote the differentiation，but CDK2 overexpression could weaken the effect of ATRA．
Keywords：retinoic acidHL—60 cellsCDK2cell cycleoverexpression
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 99-101，122 )