Construction and expression of anti--human VEGF165 ScFv
Abstract：Objective In order to reduce the immunogenicity of murine antibody.a ScFv against human VEGF165 was expressed in E-cole. Methods The variable region genes of heavy chain and light chain were amplilied by PCR. The ScFv gene was produced by overlap PCR of VH and VL,then ligated into expression vector pKpL-3a,and expressed in E.coli pop2136.The antigen binding activity of the ScFv was assayed by antigen binding ELISA and competition ELISA with the parent McAb. Resulls With the metbod of SDS-PAGE, western blot,antigen binding ELISA and competilion ELISA,it confirmed that E coli, expressed the correct 26kD of ScFv,which could specifically bind to the huaman VEGF165 and recognized the same epitope with the parent McAb. Conclusion An anti-human VEGI165 ScFV was successfully constructed expressed in E-coli.
Keywords：human VEGF155ScYvprocaryotic expression
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 92-95 )