A lung cancer associated antigen puritied by the Wheatgerm Lectin-Sepharose-6MB
Abstract：Objective To purify and characterize lung cancer associated antigen. Methods Purification by Wheat Germ Lectin-Sepharose-6MB , immunoblotting, differentail centrifigation and subcellular assay, mitotic cell immunoflourescence have been used to identify the lung cancer associated antigen. Results The lung cancer associated antigen could be purifized in large-scaie by Wheat Germ Lectin-Sepharose-6MB more convinient than N-35-Sepharose-4B.The lung cancer associated anti-gen was distributed in GLC-82,Hela,HepG-2,and PMC with different Mr,but no expression in the protein ingredient of nor-mal human heart and lung fresh tissues. It concertrated at the nuclei significantly,much more than at the mitochondrail and membrane. It was orginated at the centriole of the chromosomal domakn. Conclusion The lung cancer associated antigen N35 might be an important tumor associated antigen and related with the proliferation of cancer cells as a role of tumor cell growth regulator.
Keywords：Wheatgerm Lectin-Sepharose-6MBlung cancer associated antigensubcellular assaymitosiscentriole
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 87-91 )