Dual peak expression of transcription factor Oct-1 in apoptotic Jurkat cell line induced by diethylstilbestrol
Abstract：Objective To characterize the Jurkat cell death induced by diethylstibestrol and the 0.8g/L agarose gel elec-trophoresis expression of transcription factor Oct-1 expression in apoptotic cells. Methods DNA ladder of apoptosis was evalu-ated by 0.8g/L agarose gel electrophoresis and ethidium bromide staining,and apoptotic cells were quantified by propidium iodide(PI) staining and flow cytometry assay. Using Oct-1 specific oligo DNA probe and electrophoresis mobility shift assay(EMSA),transcription factor Oct-1 expression in the nuclei of apoptotic Jurkat cells was investigated. Results Jurkat cell apoptosis induced by diethylstilbestrol was a time and dose-dependent process. DNA ladder was noticed in apoptotic cells induced by 5mg/L of diethyistilbestrol. 30% of the Jurkat cells are dying via apoptosis after induction with 1mg/L of diethyl-stilbestrol. Dual peak transcription factor Oct-1 expression was involved in the Jurkat cell apoptosis induced by diethyl-stilbestrol.Conclusion Diethylstilbestrol could induce apoptosis of Jurkat cells in which dual peak expression of transcription faclor Oct-1 may be involved.
Keywords：diethylstilbestiolapoptosisJurkat cell lineOct-1transcription factor
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 81-83 )