Dual peak expression of transcription factor Oct-1 in apoptotic Jurkat cell line induced by diethylstilbestrol
Abstract：Objective To characterize the Jurkat cell death induced by diethylstibestrol and the 0.8g/L agarose gel eletrophoresis expression of transcription factor Oct-l expression in apoptotic cells.Methods DNA ladder of apoptosis was evalrated by 0.8g%/L agarose gel electrophoresis and ethidium bromide staining,and apoptotic cells were quantified by propidium iodide (PI) staining and flow cytometry assay .using Oct-1 speciffic oligo DNA probe and electrophoresis mobility shift assay(EMSA),transcription factor Oct-1 expression in the nuclei of apoptic Jurkat cells was investigated.results Jurkat cell apotosis induced by diethylstilbestrol was a time and dose-dependent process.DNA ladder was noticed in apopttic cells induced by 5mg/L of diethylstibestrol.30% of the Jurkat cells are dying via apoptosis after induction with lmg/L of diethylstilbestrol.Dual peak transcription.factor Oct-1 expression was involved in the Jurkat cell apoptosis induced by diethylstilbestrol.Conclusion Diethylstibestrol could induce apoptosis of Jurkat cells in which dual peak expression of transcription factor Oct-1 may be involved.
Keywords：diethylstilbestiolapoptosisJurkat cell lineOct-1transcription factor
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 81-83 )