PATHOGENICITY AND SEQUENCE ANALYSIS OF A MOUSE MONOCLONAL ANTIBODY AGAINST HUMAN ACETYLCHOLINE RECEPTOR IN MYASTHENIA GRAVIS
Abstract：The pathogenesis of myasthenia gravis(MG) on the animal model of experimental autoimmune myasthenia gravis (EAME) was investigated. The pathogenicity of a mouse monoclonal antibody against human acetylcholine receptor (AChR) was determined on the rats and the sequences of the variable regions of the antibody analysed. The anti-AChR antibody studied could induce EAMG. AChR and weight loss by 47.2 + 15.3 % and 13.4±2.2% respectively after passively transferred into rats. The heavy chain genetic element of the antibody, which showed a 94.8% homology with the most closely related germline VH, was derived from the Q52 germline family.Comparison of the variable region sequences, especially the complementarity determining regions (CDRs) with the antibody and other pathogenic anti-AChR antibodies indicates that not is the unique structure of pathogenic antiAChR antibodies needed in mediating MG and EAMG.
Keywords：Myasthenia gravisMonoclonal antibodiesAcetylcholine receptorPathogenicityNucleotide sequence
Publication Date：1999-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )