Domestication of microbially enhanced coalbed methane microorganism
Abstract：A methanogenic strain was obtained from anaerobic sludge. The strain was cultivated anaerobically with coal as solo carbon resource. The methanogenesis regulation of coal consumed by the strain and the influence of convention- al carbonaceous substrates on gas production were investigated. It is found that adaptive phase is decreased from 15 days of original strain to 6 days of acclimated strain. The gas production is greatly raised as well. A 28 days＇ gas pro- duction cycle can be divided into three phases, adaptive phase, gas-producing phase and stable phase. The total amount of gas produced by 100 mL substrate is 182 mL which comprises 16% methane. Daily gas production increases at the beginning and then decreases. During 15th day to 17th day, gas production reachs the peak amount of 20 mL/day. The strain can also make use of sodium acetate and methanol to produce gas. However, sodium acetate has a better prompt- ing effect on gas production of coal consumed by the strain.
Keywords：coalbed methanemethanogenincrease productionmicroorganism domestication
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1359-1363 )