Study on the Induction of Mulberry Callus,Germinated Seeds and Green Branches to Form Tetraploid Mulberry by Colchicine
LIU Weiqiang
XIE Zhenjiang
Abstract：In order to establish the induction system of colchicine-induced mulberry tissue to form tetraploid mulberry,the traditional mulberry variety Sha 2×Lun 109 was selected,and the callus,germinated seeds and green branches of mulberry were used as induction materials to screen the suitable concentration for colchicine-induced mulberry callus to form tetraploid mulberry,as well as the optimal concentration and soaking treatment time for colchicine-induced mulberry germinated seeds and green branches to form tetraploid mulberry.The results showed that the effect of inducing mulberry callus to form tetraploid mulberry was the best when the concentration of colchicine was 10 mg/L,and the induction rate reached 2.67%.When the concentration of colchicine was 5 mg/L and the germination seeds were soaked for 48 h,the effect of inducing tetraploid mulberry was the best,and the induction rate reached 29.00%.When the concentration of colchicine was 15 mg/L and the green branch was soaked for 24 h,the effect of inducing tetraploid mulberry was the best,and the induction rate reached 16.67%.Appropriate colchicine concentration and soaking time are of great significance for the cultivation of tetraploid mulberry varieties and polyploid breeding.
Keywords：Mulberry treeTetraploidCallus tissueGerminated seedGreen branch
Publication Date：2025-04-10
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 71-75 )