The effect of isoliquiritigenin on the proliferation,invasion and immune killing sensitivity of NK/T-cell lymphoma
LI Xiaoyan
SONG Cailu
WU Song
TANG Hailin
HUANG Guoxian
Abstract：Objective To explore the effect of isoliquiritigenin on the proliferation,invasion and immune killing sensitivity of NK/T cell lymphoma.Methods After intervention with isoliquiritigenin at multiple concentrations(0,2.5,5 and 12 µmol/L)and time points(0,12,24 and 48 h),the cell viability of NK/T cell lymphoma cells SNK-1 and SNT-8 was measured.Following intervention with different concentrations of isoliquiritigenin,clonogenic assays,flow cytometry and Transwell cell invasion experiments were used to detect changes in proliferation,apoptosis,and invasion capabilities of SNK-1 and SNT-8 cells.After extracting and isolating NK cells,the lactate dehydrogenase release assay was used to de-tect the changes in the cytotoxicity sensitivity of SNK-1 cells to NK cells before and after the treatment of Isoliquiritigenin.Flow cytometry was used to detect the expression changes of NKG2D ligands(MICA/B,ULBP1,ULBP2 and ULBP3)and HLA class I molecules on the surface of SNK-1 cells before and after the treatment of isoliquiritigenin.Results Isoliquiritigenin inhibited the proliferation of NK/T cell lymphoma SNK-1 and SNT-8 in a dose-and time-dependent man-ner,respectively(all P<0.05).Compared with the untreated group,SNK-1 and SNT-8 clone formation and cell invasion ability were weakened after treatment with isoliquiritigenin,and the apoptosis rate of cells increased(all P<0.05).At the effectual target ratios of 10∶1 and 20∶1,isoliquiritigenin could enhance the cytotoxicity sensitivity of SNK-1 cells to NK cells(all P<0.05).After the treatment of isoliquiritigenin,the expressions of MICA/B,ULBP2 and ULBP3 on the surface of SNK-1 cells was increased(all P<0.05).Conclusion Isoliquiritigenin inhibits the proliferation and invasion of NK/T cell lymphoma,promotes its apoptosis,and enhances NK cell-mediated immune killing against it.
Keywords：isoliquiritigeninNK/T cell lymphomacell proliferationcell invasionimmune killing sensitivity
Publication Date：2025-08-25
Online Publishing Date：2025-09-17(First online date of this platform, not the publication date of the document)
Pages：4( 45-48 )