The role and mechanism of circ-UBAP2 in apoptosis and inflammation of A549 cell model of acute lung injury
XUE Pingping
SUN Xinwei
LIU Xueni
WANG Wenjing
HAO Yuewei
Abstract：Objective To investigate the expression and role of circ-UBAP2 in A549 cell model of acute lung injury(ALI)and elucidate its potential molecular regulatory mechanism.Methods A549 cell was treated with LPS to construct an ALI cell model.The expression of circ-UBAP2 in cell was detected by qRT-PCR.To explore the effect of circ-UBAP2 on LPS-induced A549 cell,si-circ-UBAP2 was transfected into LPS-induced A549 cell,and then the level of inflammatory fac-tors and apoptosis were detected by ELISA and flow cytometry.Additionally,bioinformatics analysis and dual luciferase as-say were used to clarify the mechanism of circ-UBAP2.Results Circ-UBAP2 was highly expressed in LPS-induced A549 cell(P<0.01).Silencing circ-UBAP2 promoted LPS-induced apoptosis of A549 cells(P<0.01)and reduced the levels of inflammatory factors TNF-α,IL-6,IL-18 and IL-1β(all P<0.01).Circ-UBAP2 competitively bound to miR-34a-5p,and silencing circ-UBAP2 promoted the expression of miR-34a-5p in LPS-induced A549 cells(P<0.01).MiR-34a-5p targeted OGDH.Overexpression of miR-34a-5p inhibited the expression of OGDH in LPS-induced A549 cells(P<0.01).Silencing circ-UBAP2 significantly reduced the phosphorylation levels of MEK1 and MEK1/2,but the above inhibition was reversed by miR-34a-5p inhibitor.Conclusions The high expressed circ-UBAP2 in LPS-induced A549 cells can promote inflam-matory response and inhibits apoptosis.Circ-UBAP2 regulates ALI through the miR-34a-5p/OGDH/MAPK axis.
Keywords：circ-UBAP2miR-34a-5pOGDHMAPK pathwayacute lung injury
Publication Date：2025-06-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 54-58 )