Advances in the application of digital PCR technology in animal disease detection
Liu Zhiling
Huang Chao
Liang Jiaqi
Yu Haiqiong
Jiao Yanxiang
Duan Yanyu
Mei Mingzhu
Wu Xiaowei
Abstract：Pathogenic microorganisms have caused significant losses to the animal farming industry,making the development of efficient and sensitive pathogen detection technologies highly important.Digital PCR(dPCR)is a third-generation PCR detection method characterized by high accuracy,specificity,and sensitivity.It enables absolute quantification of pathogenic microorganisms without relying on standard curves and has been widely applied in the field of veterinary diagnostics.Currently,numerous studies have reported the use of dPCR for detecting pathogens such as viruses,bacteria,parasites,mycoplasmas,and chlamydia.This review summarizes the fundamental principles of dPCR technology and its applications in animal pathogen detection,aiming to provide a reference for the broader adoption of dPCR technology in the fields of animal husbandry and veterinary medicine.
Keywords：Digital PCRAnimal pathogensNucleic acid detectionAbsolute quantification
Publication Date：2026-02-15
Online Publishing Date：2026-03-16(First online date of this platform, not the publication date of the document)
Pages：6( 83-88 )