Comparison of three ELISA kits for detecting antibodies against porcine reproductive and respiratory syndrome virus
Tian Yuan
Cao Jing
Chai Wenxian
Yu Haotian
Yu Chongwei
Xia Fufang
Abstract：This study aimed to evaluate the detection performance of three commercial porcine reproductive and respiratory syndrome virus(PRRSV)antibody ELISA kits.Three negative serum samples,three positive serum samples,and 409 clinical serum samples were selected to compare the analytical sensitivity,intra-batch repeatability,and negative and positive agreement rates of the three kits.The results showed that the analytical sensitivity of Kit C is the highest,and Kits A and B are basically the same.The intra-batch coefficient of variation for Kits A,B,and C was less than 4%,indicating good repeatability across all kits.Among them,Kit B demonstrated the best repeatability,followed by Kit C.The positive agreement rates for Kits B and C were both 100.00%,while that for Kit A was 98.08%.The diagnostic sensitivity of the three kits ranked as B=C＞A.The negative agreement rate for Kit A was 100.00%,whereas those for Kits B and C were 99.59%and 97.22%,respectively.The diagnostic specificity ranked as A＞B＞C.The study indicates that Kit B achieved the highest overall performance evaluation,with excellent repeatability,high diagnostic sensitivity,and high diagnostic specificity,making it the preferred choice for antibody detection.If clinical testing prioritizes sensitivity,Kit C,which has higher analytical sensitivity and diagnostic sensitivity,may be selected.If clinical testing emphasizes specificity,Kit A,with higher diagnostic specificity,is recommended.
Keywords：PRRSVELISAAntibody detectionDetection performance
Publication Date：2026-01-15
Online Publishing Date：2026-03-05(First online date of this platform, not the publication date of the document)
Pages：5( 25-29 )