Study on the disinfection method of diluent for bovine frozen semen
Yang Lifeng
Abstract：This experiment aimed to investigate the microbial control methods for egg yolk-containing extenders used in cattle frozen semen production,as well as the effect of extender disinfection on semen performance.The study used the colony count in the frozen semen extenders,along with sperm motility before and after freezing and the sperm deformity rate after freezing,as key indicators.A comparative analysis was conducted between extenders subjected to pasteurization and untreated control groups.The results showed that the colony count in non-disinfected egg yolk-containing extenders exceeded 1 000 CFU/dose,whereas no colonies were detected in the pasteurized extenders,indicating an extremely significant disinfection effect.Pasteurization of the extenders had no significant impact on sperm motility before or after freezing,nor on the post-freezing sperm deformity rate.The study suggests that pasteurization can be widely applied in the processing of cattle frozen semen extenders.
Keywords：Bovine frozen semenPasteurizationSemen extenderColony countSperm motilitySperm abnormality rate
Publication Date：2026-01-15
Online Publishing Date：2026-03-05(First online date of this platform, not the publication date of the document)
Pages：4( 21-24 )