Analysis of the mechanism of action of Scutellaria baicalensis in inhibiting Aeromonas salmonicida based on network pharmacology and molecular docking technology
Wang Qian
Sun Yiya
Liang Xuechao
Cui Miaomiao
Xue Hongbo
Dong Xiaoping
Liu Liying
Liu Yingzi
Abstract：This research employed network pharmacology and molecular docking techniques to investigate the inhibitory mechanism of Scutellaria baicalensis against Aeromonas salmonicida.The susceptibility of Aeromonas salmonicida JMAV14 to the drug was screened through in vitro antibacterial assays of traditional Chinese medicine,and the minimum inhibitory concentration(MIC)was determined.Active components of Scutellaria baicalensis were collected from the TCMSP database,and their targets were obtained via the PubChem,Swiss Target Prediction,and Swiss ADME databases.Targets related to'Aeromonas salmonicida'were retrieved from the Genecards,OMIM,and TTD databases.Intersecting targets were identified using the Microbioinformatics Venny platform.Protein-protein interaction(PPI)analysis of the intersecting targets was performed on the STRING platform,while GO functional enrichment and KEGG pathway enrichment analyses were conducted on the DAVID platform.Molecular docking was carried out using AutoDockTools software.The results showed that 36 active components and 445 potential targets were screened from Scutellaria baicalensis,with 13 targets associated with inhibiting Aeromonas salmonicida,yielding two intersecting targets.PPI analysis revealed that Bcl2l1 and Mcl1 were core targets.GO and KEGG enrichment analyses identified 16 functional terms and three pathways related to the inhibition of Aeromonas salmonicida,with the apoptosis pathway being the most significantly affected.Both wogonin and acacetin were able to bind to the core targets MCL1 and BCL2L1.The study demonstrates that active components in Scutellaria baicalensis,such as wogonin and acacetin,act on the targets Bcl2l1 and Mcl1,inhibiting Aeromonas salmonicida by regulating the apoptosis pathway,JAK-STAT signaling pathway,and PI3K-Akt signaling pathway.
Keywords：Scutellaria baicalensisAeromonas salmonicidaNetwork pharmacologyMolecular dockingMechanism
Publication Date：2025-11-15
Online Publishing Date：2025-12-16(First online date of this platform, not the publication date of the document)
Pages：7( 18-24 )