Cloning and genetic evolution analysis of Hexon and Fiber genes of group D fowl adenovirus ZK01 strain in Zhoukou
Yin Kun
Lu Yuanlu
Liang Lu
Tian Kaiyue
Huang Yubo
Abstract：The study aimed to clarify the genetic variation of Hexon and Fiber genes of fowl adenovirus(FAdV)in Group D of a broiler farm in Zhoukou area,and to conduct PCR amplification,sequencing and sequence analysis on suspected positive broiler liver samples.The results showed that the Hexon and Fiber genes of the cloned ZK01 strain were closely related to the FAdV FJSW2021 strain of group D,and the sizes of the coding sequences(CDS)were 2 853 bp and 1 719 bp,respectively.The nucleotide homology between the ZK01 strain and the FJSW2021 strain was the highest,being 100.0%and 99.9%respectively.However,there was a significant difference from the 380 reference strains of serum type 11,with homologies of only 97.9%and 95.8%respectively,and there were multiple nonsense mutations in the Hexon gene.Studies have shown that there are group D FAdV epidemic strains in the chicken flocks in Zhoukou area,which provides certain references for the molecular epidemiological monitoring and prevention and control of FAdV in this area.
Keywords：Fowl adenovirusSerum type 11Hexon geneFiber geneGenetic evolution analysis
Publication Date：2025-11-15
Online Publishing Date：2025-12-16(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )