Research on methods for reducing endotoxin content in neonatal bovine serum
Liu Yonqging
Fu Gailing
Wu Jinlong
Zhang Jinhua
Hong Wei
Guan Yongmei
Xue Jing
Abstract：The experiment aims to establish a method for reducing the endotoxin content in newborn bovine serum.Three groups of newborn bovine serum raw materials with excessive endotoxin(>10 EU/mL)were selected for the experiment.Surfactant Triton X-100 and a mixture of montmorillonite and perlite in a specific proportion were used for step-by-step treatment and adsorption.The physicochemical indicators of newborn bovine serum(including the contents of endotoxin,hemoglobin,total protein,and molar osmotic pressure concentration,pH value)and the culture effects of cells(VERO cells,KMB-17 cells,and 293T cells)before and after treatment were compared.The results showed that this method could extremely reduce the endotoxin content in newborn bovine serum(P<0.01).The endotoxin content in the newborn bovine serum of Groups Ⅰ,Ⅱ,and Ⅲ decreased from 38.1,48.0,and 24.2 EU/mL to 2.5,4.4,and 1.7 EU/mL,respectively.There were no significant differences in the contents of hemoglobin and total protein,molar osmotic pressure concentration and pH value of newborn bovine serum before and after treatment(P>0.05).The treated neonatal bovine serum could normally culture VERO cells,KMB-17 cells and 293T cells,and the cell growth trend was consistent with that of the newborn bovine serum and the control group serum before treatment.Studies have shown that the method used in the experiment can effectively reduce the endotoxin content in newborn bovine serum,without any side effects such as destruction or reduction of the main nutrients in newborn bovine serum,and will not affect the normal growth of VERO cells,KMB-17 cells,and 293T cells.
Keywords：Newborn bovine serumEndotoxinCell culturePhysicochemical indicator
Publication Date：2025-09-15
Online Publishing Date：2025-10-28(First online date of this platform, not the publication date of the document)
Pages：8( 21-28 )