Isolation and identification of Vibrio parahaemolyticus in seafood from a market in Urumqi
Wang Hongdan
Tursun·Resalat
Ruzemai·Abudukeyoumu
Abdurahman·Abliz
Obul·Ruxangul
Aztumar·Kaderya
Maitieziz·Mamat
Abstract：This study assessed Vibrio parahaemolyticus contamination in seafood from a Urumqi market.Samples included 18 grouper tissues(heart,gills,liver,muscle,intestine,scales),20 swimming crab tissues(heart,stomach,muscle,gills),and 60 Pacific white shrimp(Litopenaeus vannamei)tissues(brain,muscle,intestinal tract).Bacterial isolation,biochemical identification,and PCR amplification targeting partial BL1S 16S rRNA gene sequences were conducted.Results indicated no Vibrio parahaemolyticus in crabs or groupers.Shrimp samples showed 30%positivity(18/60),with 23 colonies exhibiting circular,blue-green,translucent,turbid morphology and 100%biochemical confirmation(23/23).All 18 non-replicate isolates were confirmed as Vibrio parahaemolyticus,showing tissue-specific distribution:Muscle 30%(6/20),intestinal tract 15%(3/20),brain 45%(9/20).The study confirms shrimp contamination in this market,while crabs and groupers remained uncontaminated.
Keywords：Vibrio parahaemolyticusPacific white shrimp(Litopenaeus vannamei)Bacterial isolation and identificationSeafoodUrumqi
Publication Date：2025-07-15
Online Publishing Date：2025-08-22(First online date of this platform, not the publication date of the document)
Pages：5( 17-21 )