Isolation,identification,and drug resistance test of Acinetobacter baumannii from sheep in Xinjiang
Chen Xiaoxue
Chen Beibei
Wang Huixiang
Jiao Haihong
Wang Zhenxing
Abstract：This study aimed to identify the causative pathogen responsible for the disease outbreak in a sheep flock at a farm in Hotan Prefecture,Xinjiang Uygur Autonomous Region,with particular focus on the zoonotic potential of Acinetobacter baumannii.Nasal swab samples were collected from sheep exhibiting respiratory disease,leading to the isolation of one dominant bacterial strain.The isolate underwent comprehensive analysis through bacterial isolation,morphological identification,biochemical testing,16S rRNA gene sequencing,antimicrobial susceptibility testing,and mouse pathogenicity assays.Results identified the isolate as Acinetobacter baumannii(designated HT-1A),a Gram-negative,short rod-shaped bacterium.Its biochemical characteristics aligned with Acinetobacter baumannii,and 16S rRNA sequence analysis revealed 97.2%homology with Acinetobacter baumannii strains in GenBank.Antimicrobial susceptibility testing showed the isolate was susceptible only to Ceftazidime and Gentamicin.Pathogenicity testing demonstrated high virulence in mice,with a median lethal dose(LD50)of 0.6×10⁹ CFU.This study confirms Acinetobacter baumannii as the causative agent of the sheep disease outbreak,with the strain exhibiting significant pathogenicity and lethality in mice.These findings underscore Acinetobacter baumannii's role as a zoonotic pathogen and highlight the need to investigate its transmission routes between animals and humans.
Keywords：SheepAcinetobacter baumanniiBacterial isolation and identificationMice pathogenicity testZoonotic pathogen
Publication Date：2025-06-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 70-75 )