Study on the enzymatic hydrolysis effect of keratinase on bovine blood proteins
Li Jinhao
Wen Tao
Li Yan
Gao Yuhan
Lan Benxiang
Zhou Xueyan
Abstract：The study conducted research on the enzymatic hydrolysis of livestock blood to provide a reference for the rational treatment of poultry and livestock blood.Using bovine blood as the sample,keratinase was employed for blood enzymatic hydrolysis experiments.Four factors-Sample pH,enzyme dosage,hydrolysis temperature,and hydrolysis time-were investigated through single-factor and orthogonal experiments to examine their effects on the degree of hydrolysis under different enzymatic conditions and optimize the process parameters.The results showed that the optimal enzymatic hydrolysis conditions for bovine blood protein were:pH 9,enzyme dosage 3.75％,temperature 65℃,and duration 28 hours,achieving a hydrolysis degree of 49.66％ under these conditions.The study demonstrates that keratinase exhibits favorable enzymatic hydrolysis effects on bovine blood protein,providing valuable insights for rational recycling of livestock blood,reducing resource waste,and alleviating environmental pollution issues.
Keywords：KeratinaseLivestock bloodDegree of hydrolysisEnzymatic hydrolysis
Publication Date：2025-04-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 28-32 )