Structural prediction and antigenic epitope analysis of the M protein of swine acute diarrhea syndrome coronavirus
Lin Guangyu
Abstract：This study aimed to systematically reveal the molecular characteristics of the M protein of swine acute diarrhea syndrome coronavirus(SADS-CoV),providing references for vaccine design and antiviral drug development.Using bioinformatics methods,the structural and immunofunctional properties of the SADS-CoV M protein were systematically analyzed.Results showed that the SADS-CoV M protein consists of 228 amino acids,containing three transmembrane domains without a signal peptide,and exhibits hydrophobic stability.The secondary structure of the SADS-CoV M protein is predominantly composed of α-helices(32.89%)and random coils(38.60%),the predicted tertiary structure exhibits a 34.31%similarity to that of the SARS-CoV-2 M protein.Based on the tertiary structure,B-cell epitope prediction analysis was performed,identifying four high-probability B-cell epitopes(positions 13～23,65～73,80～88,and 188～195),suggesting potential targets for immune intervention.The study demonstrated that the SARS-CoV-2 M protein exhibits molecular stability and strong immunogenicity,providing a molecular foundation for the development of novel vaccines.
Keywords：Swine acute diarrhea syndrome coronavirusM proteinBioinformaticsAntigenic epitopes
Publication Date：2025-04-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 19-23 )