Biomimetic mineralization was used to improve the thermal stability of hepatitis E virus-like particles
Zhu Zhimei
Yang Shuren
Xu Yifan
Li Hui
Wang Shude
Gao Shenyang
Abstract：The study aims to enhance the thermostability of hepatitis E pseudovirus particles(MS2-HEV)using a bionically mineralized method to address the heat resistance issue of hepatitis E virus(HEV)nucleic acid testing quality control samples.The recombinant plasmid pET28b-MS2-HEV was transformed into Rosetta(DE3)supercompetent cells,and MS2-HEV was prepared after induction with isopropyl-β-D-thiogalactopyranoside(IPTG).MS2-HEV was then placed in a mineralization solution with a pH of 7.5,a final calcium ion(Ca²⁺)concentration of 5.5 mmol/L,and a calcium-to-phosphorus ratio(Ca/P)of 1.67,and mineralized at 37℃for 3 hours to prepare bionically mineralized hepatitis E pseudovirus particles(MS2-HEV-CaP).The mineralization efficiency and thermostability were analyzed using RT-qPCR and electron microscopy.The results showed that the mineralization efficiency of MS2-HEV reached 70%,and the mineralized MS2-HEV-CaP could be stably stored at 37℃for 14 days.The study demonstrated that MS2-HEV-CaP was successfully prepared,and the thermostability of MS2-HEV was improved using the bionically mineralized method.
Keywords：Hepatitis Evirus-like particlesBiomimetic mineralizationThermal stability
Publication Date：2025-04-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 8-13 )