Research progress of bovine embryo freezing methods and freezing damage in vitro
Wang Ge
Zhang Yisong
Zhang Yi
Guo Jiyuan
Wang Chaoran
Lü Xinzhe
Zhao Heping
Zhu Kai
Abstract：In vitro embryo production is an important technology for breeding fine breeds or rare protected animals.Embryo freezing can save excess embryos and their genetic material,and provide technical support for embryo transfer anytime and anywhere.Therefore,embryo freezing technology is one of the important steps before transplantation after in vitro embryo production,and it is the key to embryo transfer technology entering the commercial stage.However,due to the differences between in vitro production and natural breeding,such as the high fat content of in vitro embryos,elevated levels of reactive oxygen species,physical damage and other characteristics,the freezing effect of in vitro production embryos is not ideal.This paper mainly describes the development of in vitro embryo cryopreservation technology,characteristics of in vitro embryo,cellular molecular characteristics and cryopreservation damage after cryopreservation,in order to provide a reference for improving the technical level of in vitro embryo cryopreservation of bovine.
Keywords：CryopreservationFreezing principleIn vitro embryonic characteristicsFreezing damage
Publication Date：2025-01-14
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 85-90 )