Establishment of a method for simultaneous detection of VB2 and VB6 in premixed feed
Cao Lin
Feng Xiuyan
Lou Yingxia
Yao Ting
Jia Tao
Li Zheng
Yang Baoliang
Wang Jitong
Abstract：The aim of this experiment was to establish a high performance liquid chromatography method for simultaneous detection of VB2 and VB6 in premixed feed.The samples were extracted for 20 min in a water bath at 70℃and separated by Agilent C18 column(4.6 μm×250 mm,5 μm).The mixture of sodium heptane sulfonate,ethylenediamine tetraacetic acid(EDTA),triethylamine,acetic acid(pH 3.5)and methanol was eluted in an equal gradient ratio of 87∶13,and the diode array detector was used for on-board detection.The results showed that the linear relationship between the VB2 and VB6 was good in the range of 0.5～40.0 mg/kg(correlation coefficients greater than 0.999 9).The limit of detection and limit of quantitation of VB2 were 6.0 mg/kg,15.0 mg/kg,and the recovery was 80%～100%.The limit of detection and limit of quantitation of VB6 were 3.0 mg/kg,6.0 mg/kg,and the recovery was 90%～110%.The results show that the established method can accurately determine the content of VB2 and VB6 in the premixed feed,and has the advantages of simple,quick and time-saving.
Keywords：Premix feedVB2VB6High performance liquid chromatography
Publication Date：2024-12-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 13-17 )