Study on the effect of superovulation and embryo transfer in New Zealand rabbits

Shao Le; Qin Feng; Yang Jie; Zhai Pin; Li Jian; Pan Xiaoqing; Zhang Xia; Wang Xiaoyan

doi:10.20154/j.cnki.issn1672-9692.2024.11.003

Abstract：The aim of this study was to investigate the factors influencing the effect of hyperovulation and embryo transfer induced by different hormones in New Zealand rabbits.The experiment was conducted to investigate the effects of different hormones[follicle stimulating hormone(FSH),pregnant horse serum gonadotropin(PMSG)],season and menstrual status on the superovulation effect of female rabbit(donor),and the effects of menstrual status and synchronous estrus on the embryo transfer effect of female rabbit(recipient).The results showed that the total number of ovulation points,average number of ovulation points,total number of follicular cysts,average number of follicular cysts,total number of available embryos,average number of available embryos,number of malformed embryos and rate of embryo malformation in the experimental group Ⅰ were higher than those in the experimental group Ⅱ(P>0.05).The average number of ovulation points and the average number of available embryos in both groups were significantly higher than those in young female rabbits(P<0.05),and the average number of ovulation points and the average number of available embryos in spring were significantly higher than those in summer(P<0.05).The pregnancy rate and number of live offspring were higher than those of young female rabbits,but the difference was not significant(P>0.05).The pregnancy rate and number of live births in the light-regulated group were the highest,but there was no significant difference among the three groups(P>0.05).The results showed that the best results of pregnancy rate and number of live offspring were obtained in spring when the donor rabbits were treated with 60 IU of PMSG,the estrus was synchronized by light regulation,and the embryo transfer was performed with the recipient rabbits.